Biological indicator spore tests

Definition

Biological indicator spore tests are used to validate the efficacy of sterilization processes by using highly resistant bacterial spores. If these spores are successfully killed, it indicates that the sterilization process is effective.

5 Must Know Facts For Your Next Test

1. Biological indicator spore tests commonly use spores from Bacillus or Geobacillus species due to their high resistance.
2. These tests provide a direct measure of the lethality of the sterilization process, unlike chemical indicators which only infer conditions.
3. Spore tests are typically placed in hard-to-sterilize locations within the equipment to ensure thorough validation.
4. A successful biological indicator test means no growth is observed after incubation, indicating effective sterilization.
5. They are critical for validating autoclaves and other high-temperature sterilization methods regularly used in laboratory and clinical settings.

Review Questions

• What type of microorganisms are used in biological indicator spore tests?
• Why are biological indicator spore tests considered more reliable than chemical indicators?
• In what scenarios would you use biological indicator spore tests?

Related terms

Autoclave: A device that uses steam under pressure to achieve sterilization.

Sterility Assurance Level (SAL): The probability of a single unit being non-sterile after sterilization.

Chemical Indicator: Substances that change color or form when exposed to specific conditions, used to infer successful sterilization.