🦠microbiology review

Anaerobic jar

Written by the Fiveable Content Team • Last updated September 2025
Written by the Fiveable Content Team • Last updated September 2025

Definition

An anaerobic jar is a sealed container used to create an oxygen-free environment for the growth of anaerobic microorganisms. It typically includes chemical packets that absorb oxygen and produce carbon dioxide.

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5 Must Know Facts For Your Next Test

  1. Anaerobic jars are essential for culturing obligate anaerobes which cannot survive in the presence of oxygen.
  2. A common method to remove oxygen in an anaerobic jar involves using gas-generating sachets containing chemicals like sodium borohydride and citric acid.
  3. The effectiveness of an anaerobic jar can be verified using an indicator, such as methylene blue, which changes color in the absence of oxygen.
  4. Anaerobic jars are often used alongside other techniques like reducing media to cultivate anaerobes more effectively.
  5. These jars are crucial for studying pathogenic bacteria that thrive in low-oxygen environments, such as Clostridium species.

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