The marginal rate of technical substitution (MRTS) is the rate at which one input can be substituted for another input in the production process while keeping the output level constant. It reflects the trade-off between factors of production, showing how much of one input (like labor) must be given up to increase the other input (like capital) by a unit, maintaining the same level of production. MRTS is crucial for understanding isoquants, as it represents the slope of the isoquant curve, which illustrates different combinations of inputs that yield the same output.