Transcription Factors

Definition

Transcription factors are proteins that regulate the transcription of specific genes by binding to nearby DNA. They play a critical role in controlling gene expression, influencing cellular processes such as growth, differentiation, and response to environmental signals. Their function is essential in both prokaryotic and eukaryotic cells, and they interact with the nuclear envelope and various RNA polymerases during the transcription process.

5 Must Know Facts For Your Next Test

1. Transcription factors can function as activators or repressors, either promoting or inhibiting the transcription of target genes.
2. They often require post-translational modifications, like phosphorylation, to become fully active and to bind DNA effectively.
3. In eukaryotes, transcription factors can interact with other proteins and elements, such as enhancers and silencers, to fine-tune gene expression.
4. Different types of transcription factors include general transcription factors, which are necessary for all transcription, and specific transcription factors, which regulate specific genes.
5. The binding of transcription factors to DNA can lead to changes in chromatin structure, making the DNA more or less accessible for transcription.

Review Questions

• How do transcription factors influence gene expression during the initiation phase of transcription?
  • Transcription factors influence gene expression by binding to specific sequences in the promoter region of genes. During the initiation phase of transcription, these proteins facilitate the assembly of the transcription machinery, including RNA polymerase. By recruiting other essential proteins and forming complexes at the promoter site, transcription factors help determine whether a gene will be transcribed into mRNA.
• Discuss the differences in the roles of transcription factors in prokaryotes compared to eukaryotes.
  • In prokaryotes, transcription factors primarily bind to the promoter region and can function independently to initiate transcription. However, in eukaryotes, transcription factors have a more complex role, as they must interact with various regulatory elements like enhancers and silencers. Eukaryotic transcription also involves multiple layers of regulation, including chromatin remodeling and interactions with the mediator complex, making the control of gene expression more intricate than in prokaryotes.
• Evaluate how changes in transcription factor activity can impact cellular differentiation and development.
  • Changes in transcription factor activity can lead to significant alterations in cellular differentiation and development by modulating gene expression patterns. For instance, when specific transcription factors are activated or suppressed during development, they can direct stem cells to differentiate into specialized cell types by turning on or off sets of genes associated with those cell identities. This precise regulation is crucial for normal development; any dysregulation can result in developmental disorders or diseases such as cancer, where inappropriate gene expression patterns drive abnormal cell behavior.

Related terms

Promoter:

A region of DNA located at the start of a gene where RNA polymerase binds to initiate transcription.

Enhancer: A short region of DNA that can be bound by proteins (including transcription factors) to enhance the transcription of a gene.

Mediator Complex: A multi-protein complex that acts as a bridge between transcription factors and RNA polymerase II, facilitating the transcription process.