Termination signal

Definition

A termination signal is a specific sequence of nucleotides in DNA that indicates the end of transcription, prompting RNA polymerase to stop synthesizing RNA. This signal plays a crucial role in ensuring that RNA is produced to the correct length and that the synthesis process is properly regulated, impacting gene expression and cellular function.

5 Must Know Facts For Your Next Test

1. Termination signals can be classified into intrinsic and extrinsic signals, with intrinsic signals forming hairpin structures that cause RNA polymerase to dissociate from the DNA.
2. The presence of a termination signal is essential to prevent the unwanted transcription of adjacent genes and ensure proper gene regulation.
3. In eukaryotes, the termination process often involves additional factors, such as the recognition of specific polyadenylation signals that aid in mRNA processing.
4. Termination signals contribute to the overall efficiency of transcription by ensuring that RNA polymerase does not waste resources transcribing unnecessary sequences.
5. Mutations in termination signals can lead to read-through transcription, resulting in longer-than-normal RNA molecules that may disrupt gene function.

Review Questions

• How do intrinsic and extrinsic termination signals differ in their mechanism of action during transcription?
  • Intrinsic termination signals work by creating a hairpin loop in the RNA transcript that destabilizes the interaction between RNA polymerase and the DNA template, causing transcription to halt. In contrast, extrinsic termination relies on additional proteins or factors that bind to the RNA or DNA, facilitating the release of RNA polymerase from the transcription complex. Understanding these mechanisms helps clarify how cells regulate gene expression at the transcriptional level.
• Discuss the role of termination signals in eukaryotic transcription compared to prokaryotic transcription.
  • In eukaryotic transcription, termination signals are more complex due to additional processing steps, such as polyadenylation. Eukaryotes often require specific sequences that are recognized by proteins to ensure proper mRNA processing and stability after termination. In contrast, prokaryotic termination can occur more simply through intrinsic or extrinsic mechanisms without extensive processing. This difference highlights how eukaryotic cells have evolved more intricate systems for managing gene expression.
• Evaluate how mutations in termination signals could impact gene regulation and cellular function, citing specific examples.
  • Mutations in termination signals can lead to serious consequences for gene regulation and cellular function. For example, a mutation that disrupts a termination signal may result in read-through transcription, producing longer transcripts that can inadvertently include sequences from adjacent genes. This can lead to overexpression of certain proteins or the production of non-functional fusion proteins, ultimately affecting cellular processes like growth and differentiation. Such disruptions can contribute to various diseases, including cancer, where aberrant gene expression patterns are commonly observed.

Related terms

RNA polymerase:

An enzyme responsible for synthesizing RNA from a DNA template during the process of transcription.

promoter: A region of DNA located upstream of a gene that serves as a binding site for RNA polymerase, initiating transcription.

splicing:

The process of removing introns and joining exons together in pre-mRNA to form mature mRNA after transcription.