NADH

Definition

NADH, or nicotinamide adenine dinucleotide (in its reduced form), is a crucial coenzyme that plays a significant role in cellular respiration and metabolism. It serves as an electron carrier, transporting electrons from one reaction to another, particularly in processes like glycolysis, the citric acid cycle, and oxidative phosphorylation. By accepting and donating electrons, NADH is key in generating ATP, the energy currency of the cell.

5 Must Know Facts For Your Next Test

1. NADH is produced during glycolysis and the citric acid cycle, where it captures high-energy electrons from glucose breakdown.
2. In the electron transport chain, NADH donates electrons to initiate a series of redox reactions that ultimately lead to ATP production.
3. Each molecule of NADH can generate about 2.5 ATP molecules when oxidized in the electron transport chain.
4. Fermentation pathways can regenerate NAD+ from NADH when oxygen is scarce, allowing glycolysis to continue producing ATP.
5. The balance of NAD+/NADH ratio is critical for maintaining metabolic homeostasis and regulating various enzymatic reactions.

Review Questions

• How does NADH function as an electron carrier in cellular respiration?
  • NADH acts as an essential electron carrier by accepting electrons during metabolic reactions such as glycolysis and the citric acid cycle. Once formed, it transports these high-energy electrons to the electron transport chain. In this process, NADH donates electrons, which triggers a series of reactions that ultimately lead to ATP synthesis, showcasing its pivotal role in energy production.
• Explain the relationship between NADH and ATP production during oxidative phosphorylation.
  • During oxidative phosphorylation, NADH contributes to ATP production by donating electrons to the electron transport chain. As electrons move through the chain, they release energy that is used to pump protons across the mitochondrial membrane, creating a proton gradient. This gradient drives ATP synthase to convert ADP and inorganic phosphate into ATP, illustrating how NADH is integral to maximizing energy yield in cellular respiration.
• Evaluate the significance of NAD+/NADH balance in metabolic regulation and how this impacts cellular functions.
  • The balance between NAD+ and NADH is vital for regulating metabolic pathways and maintaining homeostasis within cells. A higher ratio of NAD+ promotes catabolic processes like glycolysis and the citric acid cycle, while an increase in NADH can signal a shift towards anabolic pathways. Disruptions in this balance can affect energy production, hinder metabolic efficiency, and contribute to various health issues, demonstrating its importance in cellular function and overall metabolism.

Related terms

NAD+: The oxidized form of NADH, which acts as an electron acceptor in metabolic reactions.

ATP: Adenosine triphosphate, the primary energy carrier in all living organisms, produced during cellular respiration.

Electron Transport Chain: A series of protein complexes in the mitochondrial membrane that transfer electrons from NADH and FADH2 to oxygen, driving ATP synthesis.