The Spanish arrival in the Southwest in 1540 marked a turning point for Native American tribes. Led by Francisco Vásquez de Coronado, Spanish conquistadors encountered Pueblos, Hopis, and Zunis, introducing new animals and attempting to establish religious footholds. Spanish colonization intensified, bringing cultural and religious clashes. Catholic missionaries sought to convert natives and suppress traditional practices. The introduction of European diseases decimated populations, while economic exploitation and resource demands reshaped Native American life.