biotechnology 3 unit 12 study guides

Key Concepts and Terminology

• Genetics studies the inheritance of traits and variations in living organisms
• DNA (deoxyribonucleic acid) stores genetic information and is composed of nucleotide base pairs (adenine, thymine, guanine, and cytosine)
• Genes are segments of DNA that code for specific proteins and determine an organism's traits
• Alleles are alternative forms of a gene that can result in different phenotypes (observable characteristics)
• Genotype refers to an organism's genetic makeup, while phenotype is the physical expression of those genes
• Chromosomes are structures that contain tightly coiled DNA and are found in the nucleus of eukaryotic cells
  • Humans have 23 pairs of chromosomes (46 total), with one set inherited from each parent
• Genome encompasses an organism's complete set of genetic material, including all genes and non-coding DNA sequences

DNA Structure and Replication

• DNA is a double-stranded helix composed of nucleotide base pairs held together by hydrogen bonds
  • Adenine (A) pairs with thymine (T), and guanine (G) pairs with cytosine (C)
• The sugar-phosphate backbone provides structural support and connects the nucleotides
• DNA replication is the process by which DNA makes an exact copy of itself during cell division
• Replication is semiconservative, meaning each new DNA molecule contains one original strand and one newly synthesized strand
• DNA polymerase is the enzyme responsible for synthesizing new DNA strands by adding nucleotides complementary to the template strand
  • DNA polymerase requires a primer (short RNA sequence) to initiate replication
• Replication occurs in the 5' to 3' direction and involves both continuous (leading strand) and discontinuous (lagging strand) synthesis
• DNA proofreading and repair mechanisms help maintain the accuracy of genetic information during replication

Gene Expression and Regulation

• Gene expression is the process by which genetic information is used to synthesize functional gene products, such as proteins
• Transcription is the first step in gene expression, where DNA is transcribed into messenger RNA (mRNA) by RNA polymerase
  • Transcription factors bind to specific DNA sequences (promoters) to initiate or regulate transcription
• Post-transcriptional modifications, such as splicing, modify the mRNA before it is translated into protein
• Translation occurs in the cytoplasm, where ribosomes read the mRNA and synthesize the corresponding amino acid sequence to form a protein
• Gene regulation controls the timing, location, and amount of gene expression in an organism
  • Regulatory mechanisms can occur at various stages, including transcriptional, post-transcriptional, translational, and post-translational levels
• Epigenetic modifications, such as DNA methylation and histone modifications, can alter gene expression without changing the DNA sequence

Mutations and Genetic Variation

• Mutations are changes in the DNA sequence that can alter gene function and lead to genetic variation
• Point mutations involve the substitution, insertion, or deletion of a single nucleotide
  • Silent mutations do not change the amino acid sequence, while missense and nonsense mutations can alter protein function
• Frameshift mutations occur when the number of nucleotides inserted or deleted is not divisible by three, shifting the reading frame
• Chromosomal mutations involve larger-scale changes, such as deletions, duplications, inversions, or translocations of DNA segments
• Mutations can be spontaneous (occurring naturally) or induced by environmental factors (mutagens) like radiation or chemicals
• Genetic variation is essential for evolution, as it provides the raw material for natural selection to act upon
  • Sources of genetic variation include mutations, recombination during meiosis, and independent assortment of chromosomes

Inheritance Patterns

• Mendelian inheritance describes the transmission of traits controlled by single genes, following dominant and recessive patterns
  • Punnett squares can be used to predict the probability of offspring inheriting specific allele combinations
• Autosomal dominant traits are expressed when an individual has at least one dominant allele (e.g., Huntington's disease)
• Autosomal recessive traits are expressed only when an individual has two recessive alleles (e.g., cystic fibrosis)
• X-linked inheritance involves genes located on the X chromosome, with different patterns for dominant and recessive traits
  • X-linked recessive traits are more common in males, as they have only one X chromosome (e.g., hemophilia)
• Codominance occurs when both alleles are expressed in the phenotype (e.g., AB blood type)
• Incomplete dominance results in a phenotype intermediate between the two alleles (e.g., pink flowers in snapdragons)
• Polygenic traits are influenced by multiple genes and often show a continuous range of phenotypes (e.g., height, skin color)

Molecular Biology Techniques

• Polymerase Chain Reaction (PCR) amplifies specific DNA sequences by using primers, DNA polymerase, and thermal cycling
  • PCR is used in various applications, such as DNA fingerprinting, genetic testing, and pathogen detection
• DNA sequencing determines the precise order of nucleotides in a DNA molecule
  • Sanger sequencing and next-generation sequencing (NGS) are common methods used for DNA sequencing
• Gel electrophoresis separates DNA, RNA, or proteins based on their size and charge by applying an electric field to a gel matrix
• Restriction enzymes are used to cut DNA at specific recognition sequences, creating fragments for analysis or cloning
• DNA cloning involves inserting a DNA fragment into a vector (e.g., plasmid) and introducing it into a host cell for replication
• Southern blotting detects specific DNA sequences by transferring DNA fragments from a gel to a membrane and hybridizing with a labeled probe
• Northern blotting is similar to Southern blotting but detects RNA instead of DNA
• Western blotting detects specific proteins using antibodies after separating them by gel electrophoresis

Applications in Biotechnology

• Recombinant DNA technology involves combining DNA from different sources to create novel genetic sequences
  • This technology is used to produce insulin, human growth hormone, and other pharmaceuticals in bacteria or yeast
• Genetically modified organisms (GMOs) have had their DNA altered using genetic engineering techniques
  • GMO crops (e.g., Bt corn) can have improved yield, nutritional content, or resistance to pests and herbicides
• Gene therapy aims to treat genetic disorders by introducing functional copies of genes into cells or modifying the expression of existing genes
  • Ex vivo gene therapy involves modifying cells outside the body and then reintroducing them, while in vivo gene therapy directly delivers genes to cells within the body
• Personalized medicine uses an individual's genetic information to tailor medical treatments and preventive strategies
  • Pharmacogenomics studies how genetic variations influence drug response and helps optimize medication dosing and selection
• Forensic DNA analysis uses genetic markers to identify individuals or establish familial relationships in criminal investigations or paternity cases
• Synthetic biology involves designing and constructing new biological systems or organisms with specific functions
  • Applications include biofuel production, biosensors, and novel materials

Ethical Considerations and Future Directions

• Genetic testing raises concerns about privacy, discrimination, and the psychological impact of knowing one's genetic predispositions
  • Genetic counseling helps individuals understand and adapt to the medical and familial implications of genetic contributions to disease
• Gene editing technologies, such as CRISPR-Cas9, allow precise modifications to DNA sequences but raise ethical questions about their use in humans
  • Germline gene editing, which affects future generations, is particularly controversial due to potential unintended consequences and the risk of exacerbating social inequalities
• Bioethics addresses the moral and societal implications of biotechnology, considering principles such as autonomy, beneficence, non-maleficence, and justice
• Intellectual property rights and patents on genetic sequences or engineered organisms can impact research, innovation, and access to biotechnology products
• International collaboration and regulation are essential to ensure responsible development and deployment of biotechnologies
• Future advancements in biotechnology may include more targeted gene therapies, improved precision medicine, and the development of novel biomaterials and biofuels
  • Integrating artificial intelligence and machine learning with biotechnology could accelerate drug discovery and personalized treatment strategies