key term - Resist changes in pH

Definition

To resist changes in pH means the ability of a solution to maintain a relatively stable acidity or basicity despite the addition of acids or bases. This characteristic is primarily exhibited by buffer solutions, which consist of a weak acid and its conjugate base or a weak base and its conjugate acid. Buffers work by neutralizing added acids or bases, thus keeping the pH within a narrow range, which is crucial for many biological and chemical processes.

5 Must Know Facts For Your Next Test

1. Buffers typically contain a weak acid and its conjugate base, or a weak base and its conjugate acid, which helps to resist pH changes.
2. The capacity of a buffer to resist pH changes depends on the concentrations of the weak acid and its conjugate base present in the solution.
3. Buffer solutions are essential in biological systems where enzymes and biochemical reactions require specific pH ranges to function optimally.
4. When strong acids or bases are added to a buffer, the weak acid or base reacts with them to neutralize their effect, thus stabilizing the pH.
5. The Henderson-Hasselbalch equation can be used to calculate the exact pH of buffer solutions by relating it to the ratio of the concentrations of the buffer components.

Review Questions

• How do buffer solutions work to resist changes in pH when strong acids or bases are introduced?
  • Buffer solutions resist changes in pH by containing a weak acid and its conjugate base (or vice versa) that react with any added strong acids or bases. When an acid is added, the weak base in the buffer will neutralize it, forming more of the weak acid. Conversely, if a base is added, the weak acid will react with it to form more of the conjugate base. This process allows buffers to minimize shifts in pH, maintaining stability in the solution.
• What is the significance of the Henderson-Hasselbalch equation in understanding how buffers resist changes in pH?
  • The Henderson-Hasselbalch equation provides a quantitative way to understand how buffers maintain stable pH levels. By relating pH to the ratio of concentrations of a weak acid and its conjugate base, this equation allows for predictions about how changing these concentrations will affect the overall pH. This understanding is crucial for applications in biochemistry and medicine where maintaining optimal pH levels is necessary for various physiological processes.
• Evaluate how buffer capacity can influence experimental outcomes in biochemical assays.
  • Buffer capacity significantly influences experimental outcomes because it determines how well a solution can maintain its pH during reactions that may produce acids or bases. In biochemical assays, if the buffer capacity is too low, even small amounts of reactants can lead to drastic pH shifts that may inhibit enzyme activity or alter reaction kinetics. Therefore, selecting appropriate buffers with adequate capacity ensures that experiments yield reliable and reproducible results by maintaining stable conditions throughout.

Related terms

Buffer solution: A buffer solution is a system that minimizes pH changes upon the addition of small amounts of acids or bases, typically composed of a weak acid and its conjugate base or a weak base and its conjugate acid.

Henderson-Hasselbalch equation:

The Henderson-Hasselbalch equation relates the pH of a buffer solution to the concentration of the acid and its conjugate base, allowing for the calculation of pH in biochemical and physiological contexts.

Weak acid: A weak acid is an acid that partially dissociates in solution, establishing an equilibrium between the undissociated acid and its ions, and plays a key role in buffer systems.