🔬general biology i review

key term - Stop codon

Definition

A stop codon is a nucleotide triplet within mRNA that signals the termination of protein synthesis during translation. It does not code for any amino acid and serves as a crucial signal for the ribosome to release the newly formed polypeptide chain. The presence of stop codons ensures that proteins are synthesized to their correct lengths, preventing the addition of extra, unnecessary amino acids.

5 Must Know Facts For Your Next Test

  1. There are three stop codons in the genetic code: UAA, UAG, and UGA, each of which signals the end of protein synthesis.
  2. Stop codons do not have corresponding tRNA molecules, meaning they do not add an amino acid to the growing polypeptide chain.
  3. The recognition of a stop codon by the ribosome triggers the recruitment of release factors, which facilitate the disassembly of the ribosomal complex.
  4. Stop codons play a critical role in ensuring that proteins are synthesized accurately and efficiently, contributing to proper cellular function.
  5. Mutations that affect stop codons can lead to diseases or malfunctioning proteins due to either premature termination or read-through effects.

Review Questions

  • How do stop codons contribute to the accuracy of protein synthesis?
    • Stop codons ensure that protein synthesis is accurate by signaling the ribosome when to terminate translation. Without these signals, ribosomes could continue adding amino acids beyond the intended endpoint, leading to dysfunctional proteins. This precise control over protein length is essential for maintaining proper cellular functions and preventing potential diseases caused by improperly synthesized proteins.
  • Discuss the role of release factors in relation to stop codons during translation.
    • Release factors are crucial proteins that recognize stop codons during translation. When a stop codon appears in the A site of the ribosome, release factors bind to it instead of a tRNA. This binding triggers the hydrolysis of the bond between the polypeptide chain and the tRNA in the P site, leading to the release of the completed polypeptide and disassembly of the ribosomal complex, thus concluding the translation process.
  • Evaluate how mutations affecting stop codons can influence protein function and overall cellular health.
    • Mutations that alter or create new stop codons can have serious implications for protein function. If a mutation introduces a premature stop codon, it may result in truncated proteins that are unable to perform their intended functions. Conversely, mutations that cause read-through of stop codons can lead to elongated proteins with incorrect amino acid sequences. Both scenarios can disrupt normal cellular processes and may lead to diseases such as cancer or genetic disorders, highlighting the critical importance of precise regulation in protein synthesis.