🧬Biochemistry Unit 18 – Amino Acid Metabolism

Key Concepts and Definitions

• Amino acids are organic compounds containing an amino group, a carboxyl group, and a side chain (R group) that varies between different amino acids
• Proteins are macromolecules composed of amino acids linked together by peptide bonds
• Metabolism refers to the chemical reactions involved in maintaining the living state of cells and organisms
• Catabolism breaks down complex molecules into simpler ones, releasing energy in the process
• Anabolism builds complex molecules from simpler ones, requiring an input of energy
• Transamination is the transfer of an amino group from one molecule to another, often involving the cofactor pyridoxal phosphate (PLP)
• Deamination removes the amino group from an amino acid, releasing ammonia ($NH_3$) as a byproduct

Amino Acid Structure and Properties

• Amino acids have a central carbon atom (α-carbon) bonded to an amino group ($-NH_2$), a carboxyl group ($-COOH$), a hydrogen atom, and a variable side chain (R group)
• The R group determines the unique properties of each amino acid, such as polarity, charge, and hydrophobicity
  • Polar amino acids (serine, threonine) are hydrophilic and often found on the surface of proteins
  • Non-polar amino acids (leucine, valine) are hydrophobic and typically buried within the protein structure
• Amino acids can be classified as acidic (aspartic acid, glutamic acid), basic (lysine, arginine), or neutral (alanine, glycine) based on their side chain properties
• The ionization state of amino acids depends on the pH of the environment, with the isoelectric point (pI) being the pH at which the amino acid has a net neutral charge
• Amino acids can exist in two mirror-image forms called L- and D-stereoisomers, with L-amino acids being the predominant form in nature

Protein Synthesis and Breakdown

• Protein synthesis occurs through the process of translation, where the genetic information in mRNA is decoded to produce a specific sequence of amino acids
• Ribosomes are the cellular organelles responsible for protein synthesis, consisting of two subunits (large and small) that assemble on the mRNA
• tRNAs (transfer RNAs) are adapter molecules that carry specific amino acids to the ribosome and base pair with the corresponding codons on the mRNA
• The genetic code is the set of rules that defines the relationship between codons (triplets of nucleotides) and the amino acids they specify
  • The code is degenerate, meaning that multiple codons can code for the same amino acid
  • Start codons (AUG) initiate translation, while stop codons (UAA, UAG, UGA) terminate it
• Protein breakdown (proteolysis) is catalyzed by enzymes called proteases, which hydrolyze peptide bonds between amino acids
• Ubiquitin-mediated proteolysis is a regulated pathway that targets specific proteins for degradation by tagging them with ubiquitin molecules

Essential vs. Non-Essential Amino Acids

• Essential amino acids cannot be synthesized by the body and must be obtained through the diet
  • There are nine essential amino acids in humans: histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine
• Non-essential amino acids can be synthesized by the body from other amino acids or precursor molecules
  • Examples include alanine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, proline, serine, and tyrosine
• Conditionally essential amino acids are usually non-essential but may become essential under certain conditions (stress, illness, or limited nutrient availability)
  • Arginine, cysteine, glutamine, tyrosine, and glycine are considered conditionally essential in some situations
• A balanced diet containing a variety of protein sources is necessary to ensure an adequate supply of all essential amino acids for proper growth and development

Amino Acid Catabolism Pathways

• Amino acid catabolism involves the breakdown of amino acids to generate energy or to serve as precursors for the synthesis of other molecules
• Transamination is the first step in the catabolism of most amino acids, transferring the amino group to α-ketoglutarate to form glutamate
  • Glutamate can then undergo oxidative deamination by glutamate dehydrogenase to release ammonia and regenerate α-ketoglutarate
• The carbon skeletons of amino acids are converted into metabolic intermediates that can enter the citric acid cycle (TCA cycle) for energy production
  • Glucogenic amino acids (alanine, cysteine, glycine, serine) can be converted into glucose via gluconeogenesis
  • Ketogenic amino acids (leucine, lysine) can be converted into ketone bodies or fatty acids
• Some amino acids have unique degradation pathways, such as the branched-chain amino acids (leucine, isoleucine, valine) which are catabolized by a shared set of enzymes
• Disorders of amino acid metabolism can lead to the accumulation of toxic intermediates, as seen in phenylketonuria (PKU) where phenylalanine and its byproducts build up due to a deficiency in phenylalanine hydroxylase

Nitrogen Balance and Excretion

• Nitrogen balance refers to the difference between nitrogen intake (from protein) and nitrogen excretion (as urea, ammonia, and other nitrogenous compounds)
  • Positive nitrogen balance occurs when intake exceeds excretion, indicating a state of growth or tissue repair
  • Negative nitrogen balance occurs when excretion exceeds intake, indicating a state of protein breakdown or starvation
• Ammonia ($NH_3$) is a toxic byproduct of amino acid catabolism that must be efficiently removed from the body
  • In the liver, ammonia is converted into urea through the urea cycle, a series of enzymatic reactions that incorporate carbon dioxide and aspartate
  • Urea is a water-soluble molecule that can be safely excreted in the urine by the kidneys
• Glutamine plays a key role in ammonia transport and detoxification, serving as a carrier of ammonia between tissues
  • In the kidneys, glutamine is deaminated to release ammonia, which helps to maintain acid-base balance by buffering excess protons ($H^+$) in the urine
• Disorders of the urea cycle, such as ornithine transcarbamylase (OTC) deficiency, can lead to hyperammonemia and neurological damage if left untreated

Metabolic Disorders and Diseases

• Inborn errors of amino acid metabolism are genetic disorders caused by defects in enzymes involved in amino acid catabolism or synthesis
  • Phenylketonuria (PKU) is caused by a deficiency in phenylalanine hydroxylase, leading to the accumulation of phenylalanine and its toxic byproducts
  • Maple syrup urine disease (MSUD) is caused by a defect in the branched-chain α-keto acid dehydrogenase complex, resulting in the buildup of branched-chain amino acids and their corresponding α-keto acids
• Nutritional deficiencies can also impact amino acid metabolism, such as kwashiorkor, a form of protein-energy malnutrition characterized by edema, liver dysfunction, and impaired growth
• Certain cancers, such as hepatocellular carcinoma, can alter amino acid metabolism by upregulating glutamine utilization to support rapid cell proliferation
• Metabolic diseases like diabetes and obesity can affect amino acid metabolism by altering insulin signaling and glucose homeostasis, leading to changes in protein turnover and nitrogen balance

Clinical and Research Applications

• Amino acid analysis is used in the diagnosis and monitoring of inborn errors of metabolism, by measuring the levels of specific amino acids in blood or urine samples
  • Newborn screening programs often include tests for PKU, MSUD, and other amino acid disorders to enable early detection and intervention
• Nutritional support for critically ill patients often involves the use of specialized amino acid formulations to promote wound healing, immune function, and muscle preservation
  • Branched-chain amino acid supplementation has been investigated as a potential therapy for liver disease, muscle wasting, and exercise recovery
• Amino acid tracers, such as stable isotope-labeled amino acids, are used in research to study protein synthesis and breakdown rates in various tissues and conditions
  • Measuring the incorporation of labeled amino acids into proteins can provide insights into the effects of aging, exercise, and disease on protein metabolism
• Targeted amino acid therapies are being developed for certain cancers, aiming to exploit the unique metabolic dependencies of tumor cells
  • Glutaminase inhibitors, which block the conversion of glutamine to glutamate, have shown promise in preclinical studies of various cancer types
• Dietary interventions focused on amino acid intake, such as low-protein diets for chronic kidney disease or high-protein diets for weight management, are an active area of research and clinical practice