Genomics
Sanger sequencing is a method for determining the nucleotide sequence of DNA, developed by Frederick Sanger in the 1970s. This technique involves selectively incorporating chain-terminating dideoxynucleotides during DNA replication, which allows for the identification of the sequence based on the lengths of the resulting fragments. As a foundational method in genomics, Sanger sequencing played a crucial role in the Human Genome Project and is still widely used today for sequencing small regions of DNA and validating results from next-generation sequencing technologies.
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