1. Scientists studied two temperate freshwater lakes to investigate how nutrient availability affects ecosystem productivity and food web structure. Lake A is oligotrophic with low nutrient concentrations, while Lake B is mesotrophic with moderate nutrient concentrations. Both lakes are located in the same climate region and have similar physical characteristics including depth, surface area, and temperature profiles.
Figure 1. Nitrogen and phosphorus concentrations (mg/L) in two temperate freshwater lakes (Lake A oligotrophic; Lake B mesotrophic). Each lake displays two adjacent bars: total nitrogen and total phosphorus; vertical error bars show ±1 standard error.
Figure 2. Biomass (g/m²) at four trophic levels in Lake A versus Lake B. Each trophic level displays two adjacent bars (Lake A and Lake B); vertical error bars show ±1 standard error.
A group of students designed an investigation to test how phosphorus availability affects algal growth in freshwater ecosystems. They collected water samples from an oligotrophic lake and divided the water equally into 20 identical glass containers. They added different concentrations of phosphorus to the containers: 5 containers received 0 mg/L (control), 5 received 0.01 mg/L, 5 received 0.02 mg/L, and 5 received 0.03 mg/L. All containers were placed under identical light conditions at 20°C for 14 days. Students measured algal biomass using chlorophyll-a concentration at the end of the experiment.
i. Identify the independent variable in the students' investigation.
ii. Describe one reason why the students used multiple containers (replicates) for each phosphorus concentration rather than using only one container per concentration.
The students' investigation showed that algal biomass increased with phosphorus concentration up to 0.02 mg/L, but showed no additional increase at 0.03 mg/L.
i. Explain why algal biomass did not continue to increase beyond 0.02 mg/L phosphorus concentration, even though more phosphorus was available.
ii. Explain how the results of the investigation would likely differ if the students had conducted the experiment using water from Lake B instead of water from an oligotrophic lake.
Oligotrophic lakes like Lake A typically have high dissolved oxygen concentrations throughout the water column, while eutrophic lakes often experience hypoxic (low oxygen) conditions in deeper waters during summer months.