Fiveable
♻️AP Environmental Science
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♻️AP Environmental Science

FRQ 1 – Experimental Design
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Unit 1: The Living World: Ecosystems
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Practice FRQ 1 of 221/22
1. Phytoplankton are microscopic photosynthetic organisms that form the base of aquatic food webs. In freshwater lakes, phytoplankton populations are influenced by nutrient availability, particularly nitrogen and phosphorus. These nutrients enter lakes through surface runoff from surrounding watersheds.
A. Describe one step in the nitrogen cycle that converts atmospheric nitrogen (N2\text{N}_2N2​) into a form that can be used by phytoplankton.
B. Explain how an increase in phytoplankton populations in Lake A could affect the dissolved oxygen levels available to fish populations at night.

Figure 1. Average Nutrient Concentrations in Lake A and Lake B (mean ± standard error).

Grouped bar graph with two lake groups (Lake A and Lake B), each showing two bars: Nitrogen and Phosphorus.

Axes and layout (must be drawn exactly as specified):
- X-axis label centered below axis: "Lake".
- X-axis has exactly two category tick marks with labels directly under the ticks: "Lake A" and "Lake B".
- X-axis tick positions are fixed as: Lake A centered at x-position 1; Lake B centered at x-position 2. (These x-position numbers are NOT printed as text; only the category labels appear.)
- Y-axis label rotated vertically: "Nutrient concentration (mg/L)".
- Y-axis numeric range: from 0 to 2.5 mg/L, inclusive.
- Y-axis tick marks and labels at exactly these values: 0, 0.5, 1.0, 1.5, 2.0, 2.5.
- The origin at the lower-left corner is explicitly labeled with the y-axis tick value "0".
- No gridlines.
- No title text inside the plotting area (caption is outside the plot).
- Axis style: no arrows on the axes; axes end flush at the plotting boundary.

Bar geometry and styling (uniform across all bars):
- Each lake group contains two adjacent bars centered symmetrically around the lake’s x-position.
- Uniform bar width for all four bars: 0.28 x-axis units.
- Within each lake group: the Nitrogen bar is the left bar; the Phosphorus bar is the right bar.
- Gap between the two bars within a group: 0.08 x-axis units (empty space between the bar edges).
- Bar fill colors:
  - Nitrogen bars: solid dark gray fill.
  - Phosphorus bars: solid light gray fill.
- All bars have a solid black outline with medium stroke thickness.

Exact bar heights (means):
- Lake A, Nitrogen (left bar in Lake A group): bar top aligns exactly with 2.2 mg/L.
- Lake A, Phosphorus (right bar in Lake A group): bar top aligns exactly with 0.8 mg/L.
- Lake B, Nitrogen (left bar in Lake B group): bar top aligns exactly with 0.4 mg/L.
- Lake B, Phosphorus (right bar in Lake B group): bar top aligns exactly with 0.1 mg/L.

Error bars (present on EVERY bar; non-zero; drawn as vertical lines with horizontal caps):
- Error bars are centered horizontally on each bar.
- Error bar line color: black; same stroke thickness as bar outlines.
- Error bar cap width (horizontal cap length): 0.18 x-axis units.
- Lake A, Nitrogen: mean 2.2 mg/L with error bar endpoints exactly at 2.0 mg/L and 2.4 mg/L.
- Lake A, Phosphorus: mean 0.8 mg/L with error bar endpoints exactly at 0.72 mg/L and 0.88 mg/L.
- Lake B, Nitrogen: mean 0.4 mg/L with error bar endpoints exactly at 0.36 mg/L and 0.44 mg/L.
- Lake B, Phosphorus: mean 0.1 mg/L with error bar endpoints exactly at 0.09 mg/L and 0.11 mg/L.

Legend (must be present):
- A legend box in the upper-right of the plotting area.
- Legend entries:
  - Dark gray swatch labeled "Nitrogen".
  - Light gray swatch labeled "Phosphorus".
C. Based on the data in Figure 1, identify the phosphorus concentration in Lake B.

Figure 2. Gross Primary Productivity (GPP) in Lake A and Lake B Over Time.

Two-line time-series graph showing gross primary productivity for two lakes across the 12 months of a single year.

Axes (must be drawn exactly as specified):
- X-axis label centered below axis: "Month".
- X-axis range: January through December, displayed as 12 evenly spaced categorical ticks.
- X-axis tick labels (exact text, left to right): "Jan", "Feb", "Mar", "Apr", "May", "Jun", "Jul", "Aug", "Sep", "Oct", "Nov", "Dec".
- X-axis has a numeric baseline with the leftmost tick at Jan and rightmost tick at Dec; tick spacing is uniform.
- Y-axis label rotated vertically: "GPP (g C/m²/day)".
- Y-axis numeric range: 0 to 8.
- Y-axis tick marks and labels at exactly these values: 0, 1, 2, 3, 4, 5, 6, 7, 8.
- The origin at the lower-left corner is labeled with the y-axis tick value "0".
- Arrows on positive ends of both axes (right end of x-axis and top end of y-axis).
- No gridlines.

Line styles (must be visually distinct and consistent):
- Lake A: solid black line, medium thickness.
- Lake B: dashed black line, same thickness as Lake A.
- Data markers: use small filled circles at each month for both lines (same marker size); markers are black for both lines.

Exact monthly values (each marker must sit exactly at the stated y-value for that month):
Lake A (solid line):
- Jan: 2.5
- Feb: 2.9
- Mar: 3.8
- Apr: 4.8
- May: 5.6
- Jun: 6.5
- Jul: 7.2
- Aug: 6.1
- Sep: 5.1
- Oct: 4.2
- Nov: 3.4
- Dec: 2.8

Lake B (dashed line):
- Jan: 1.2
- Feb: 1.4
- Mar: 1.9
- Apr: 2.4
- May: 2.7
- Jun: 3.1
- Jul: 3.5
- Aug: 2.9
- Sep: 2.2
- Oct: 1.9
- Nov: 1.6
- Dec: 1.4

Curve/segment behavior (describe how the line connects markers):
- Between each pair of adjacent months, connect markers with straight line segments (polyline), not a smoothed curve.
- Lake A polyline behavior: from Jan to Jul it increases every month (strictly upward each segment), then from Jul to Dec it decreases from Jul to Nov (strictly downward each segment) and slightly increases from Nov to Dec (upward segment).
- Lake B polyline behavior: from Jan to Jul it increases every month (strictly upward each segment), then from Jul to Dec it decreases every month (strictly downward each segment).
- The single highest point for each lake is at Jul (Lake A at 7.2; Lake B at 3.5).

Legend (must be present):
- Legend box inside plotting area near the upper-left.
- Legend entries:
  - Solid line sample labeled "Lake A".
  - Dashed line sample labeled "Lake B".

No additional annotations:
- Do not print the numeric y-values next to the points; only show the axis tick labels and the legend.
D. Based on the data in Figure 2, describe the trend in gross primary productivity in Lake A from January through July.
E. Scientists hypothesized that lakes receiving agricultural runoff would have higher gross primary productivity than lakes surrounded by forest. Describe one way that the data in Figure 2 support this hypothesis.
F.
Phytoplankton diversity is an important indicator of ecosystem health. A group of students wanted to investigate factors that affect phytoplankton diversity in local ponds. They selected two study sites: Pond X, which receives runoff from a parking lot, and Pond Y, which is located in a nature preserve. The students collected 500 mL water samples from 8 randomly selected locations in each pond. They used microscopes to identify and count the number of different phytoplankton species in each sample.
i. Identify a likely scientific question for the students' investigation of phytoplankton diversity.
ii. Identify the independent variable in the students' investigation.
LakeSpecies 1Species 2Species 3Species 4Species 5Species 6Species 7
Lake AXXX
Lake BXXXXXX
G.
The data from the student investigation of phytoplankton diversity are shown in the following table. An 'X' in the table indicates that the species was present at that site.
i. Explain why the phytoplankton community in Lake B would be more resilient to an environmental disturbance, such as a sudden temperature increase, than the phytoplankton community in Lake A would.
ii. Explain how the energy transfer efficiency from phytoplankton to zooplankton would be affected if only 8% of the energy at the phytoplankton trophic level was transferred to the zooplankton trophic level instead of the typical 10%.
Eutrophication is a common problem in lakes that receive excess nutrients from agricultural runoff.
H. Describe one negative effect that eutrophication can have on fish populations in a lake ecosystem.






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