Philosophy of Biology
Bisulfite sequencing is a method used to determine the methylation status of DNA, allowing researchers to analyze epigenetic modifications. This technique involves treating DNA with sodium bisulfite, which converts unmethylated cytosines to uracils while leaving methylated cytosines unchanged, thus enabling the differentiation between these two forms during subsequent sequencing. This process is crucial for understanding how epigenetic changes influence gene expression and can be passed down through generations.
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