Repetitive sequence-based PCR

Definition

Repetitive sequence-based PCR (rep-PCR) is a molecular technique used to amplify and analyze repetitive DNA sequences in bacterial genomes. It helps in the genotyping and differentiation of microbial strains by generating unique fingerprint patterns.

5 Must Know Facts For Your Next Test

1. Rep-PCR targets repetitive elements such as REP, ERIC, or BOX sequences in bacterial DNA.
2. It generates distinct banding patterns on agarose gels that can be used for strain identification.
3. Rep-PCR is commonly used in epidemiological studies to track microbial outbreaks.
4. The technique requires specific primers that anneal to the repetitive sequences within the genome.
5. Results from rep-PCR are analyzed using software to compare genetic similarities among different samples.

Review Questions

• What types of repetitive elements does rep-PCR target in bacterial genomes?
• How is rep-PCR useful in epidemiological studies?
• What is the role of primers in rep-PCR?

Related terms

PCR (Polymerase Chain Reaction): A technique used to amplify specific DNA sequences.

Genotyping: The process of determining differences in the genetic make-up or genotype of an individual by examining their DNA sequence.

Agarose Gel Electrophoresis: A method used to separate DNA fragments based on size through a gel matrix.