Most probable number (MPN) method
from class: Microbiology Definition The Most Probable Number (MPN) method is a statistical technique used to estimate the number of viable microorganisms in a sample. It is particularly useful for samples with low concentrations of bacteria.
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Predict what's on your test 5 Must Know Facts For Your Next Test MPN is based on the principle of dilution and relies on probability theory. It involves inoculating multiple tubes with serial dilutions of a sample and incubating them to observe microbial growth. Results are interpreted by comparing the number of positive tubes at each dilution level against standard MPN tables. The MPN method is commonly used for water quality testing, especially to detect coliform bacteria. One advantage of MPN over direct counting methods is its ability to detect very low levels of microorganisms. Review Questions What principle does the Most Probable Number (MPN) method rely on? In what scenario would you prefer using the MPN method over direct counting methods? How are results from an MPN test typically interpreted?
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