Written by the Fiveable Content Team • Last updated September 2025
Written by the Fiveable Content Team • Last updated September 2025
Definition
An immunoblot, also known as a Western blot, is a laboratory method used to detect specific proteins in a sample using antibody binding. It is commonly used to analyze the immune response and protein expression.
5 Must Know Facts For Your Next Test
Immunoblots involve separating proteins by gel electrophoresis before transferring them to a membrane.
Primary antibodies are used to specifically bind to the target protein on the membrane.
Secondary antibodies, which are often conjugated with enzymes or fluorescent markers, bind to primary antibodies for detection.
Commonly used detection methods include chemiluminescence and colorimetric assays.
Blocking agents like non-fat milk or BSA are used to prevent non-specific binding of antibodies.