Far-western blots

Definition

Far-western blots are a type of protein detection method that identifies protein-protein interactions using labeled proteins as probes instead of antibodies. This technique is particularly useful in studying the functional aspects of proteins in biological pathways.

5 Must Know Facts For Your Next Test

1. Far-western blots involve the use of a labeled 'bait' protein to detect its interacting 'prey' protein on a membrane.
2. Unlike traditional Western blots, Far-western blots do not rely on antibodies for detection.
3. This method can be used to study complex protein interactions and signaling pathways in cells.
4. The process involves running proteins on SDS-PAGE, transferring them to a membrane, and then probing with the labeled bait protein.
5. Detection usually involves chemiluminescence or autoradiography to visualize the interacting proteins.

Review Questions

Related terms

Western Blot:

A technique used to detect specific proteins in a sample using antibodies.

SDS-PAGE:

A method for separating proteins based on their electrophoretic mobility (size).

Chemiluminescence:

A detection method that uses light emission from a chemical reaction to identify target molecules.