Stokes Shift refers to the difference in wavelength between the peak absorption and the peak emission of a fluorescent molecule. This phenomenon is crucial in understanding how biological systems utilize fluorescence and phosphorescence for various functions, as it allows for clear detection of emitted light after a molecule absorbs energy. The concept plays an essential role in fluorescence spectroscopy, where the shift helps distinguish between excitation and emission wavelengths, making it easier to analyze biological samples.
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