---
title: "AP Biology 1.3: Biological Macromolecules"
description: "Review AP Biology 1.3, including monomers, polymers, dehydration synthesis, hydrolysis, polymerization, enzymes, and why lipids are not true polymers."
canonical: "https://fiveable.me/ap-bio/unit-1/intro-biological-macromolecules/study-guide/GbUEgZQ9FSaSLCMi5Emo"
type: "study-guide"
subject: "AP Biology"
unit: "Unit 1 – Chemistry of Life"
lastUpdated: "2026-06-09"
---

# AP Biology 1.3: Biological Macromolecules

## Summary

Review AP Biology 1.3, including monomers, polymers, dehydration synthesis, hydrolysis, polymerization, enzymes, and why lipids are not true polymers.

## Guide

Big biological molecules are built and broken down through two opposite reactions. [Dehydration synthesis](/ap-bio/key-terms/dehydration-synthesis "fv-autolink") links [monomers](/ap-bio/key-terms/monomer "fv-autolink") into polymers by removing the equivalent of a water molecule, and hydrolysis splits polymers back into monomers by adding water. These reactions explain how cells make and recycle carbohydrates, proteins, and nucleic acids, while lipids use related chemistry but are not true polymers.

## Why This Matters for the AP Biology Exam

This topic gives you the chemistry behind every macromolecule you study later in [Unit 1](/ap-bio/unit-1 "fv-autolink") and beyond. You will use it to describe how monomers connect into polymers, explain what happens to water during building and breaking reactions, and predict how cells store and release [energy](/ap-bio/unit-3/environmental-impacts-on-enzyme-function/study-guide/Q8PevM3BI76060aoWtit "fv-autolink") when they make or digest large molecules.

On the [AP Biology](/ap-bio "fv-autolink") exam, this shows up in multiple-choice questions about reaction types and in evidence-based written responses where you describe processes and connect structure to function. Getting the vocabulary exactly right matters, since mixing up terms like dehydration synthesis and hydrolysis can cost you points.

## Key Takeaways

- Monomers are small subunits; polymers are long chains of monomers joined by [covalent bonds](/ap-bio/key-terms/covalent-bonds "fv-autolink").
- Dehydration synthesis builds polymers by removing an H from one monomer and an OH from another, releasing the equivalent of one water molecule per bond formed.
- Hydrolysis breaks polymers by adding water, putting the H on one monomer and the OH on the other.
- Building reactions are anabolic and require energy and [enzymes](/ap-bio/unit-3/enzyme-structure/study-guide/jsjBfuk2jmYAZVrmVwtF "fv-autolink"); breaking reactions are catabolic.
- Polymerization is just dehydration synthesis repeated to link many monomers.
- Lipids are not true polymers, so they are assembled and broken down with related but distinct chemistry rather than the same monomer-to-polymer reactions.

## Polymers and Monomers

A **monomer** is a small building-block molecule. A **polymer** is a long molecule made of many monomers bonded together covalently (poly means many, mono means one).

Three of the four main macromolecule groups are true polymers: carbohydrates (from [monosaccharides](/ap-bio/key-terms/monosaccharides "fv-autolink")), proteins (from amino acids), and nucleic acids (from nucleotides). Lipids are the exception. They do not usually form polymers, so they are not built and broken down the same way the polymer [macromolecules](/ap-bio/unit-1/elements-life/study-guide/kLZ8GN081XmAmZpivYFN "fv-autolink") are.

In the polymer macromolecules, monomers are linked by covalent bonds. Those bonds are formed during dehydration synthesis and broken during hydrolysis.

## Dehydration Synthesis and Hydrolysis

These are two opposite reactions. One builds, one breaks, and water is the key player in both.

**Dehydration synthesis** joins two smaller molecules by forming a [covalent bond](/ap-bio/key-terms/covalent-bond "fv-autolink") between them. An H is removed from one monomer and an OH is removed from the other, which together make the equivalent of one water molecule (dehydrate means water is lost). Repeat this reaction many times and you link many monomers into a polymer, a process called **polymerization**. Dehydration synthesis is a [condensation](/ap-bio/unit-8/energy-flow-through-ecosystems/study-guide/A1PeQD1Zy3BIMu1zSMzd "fv-autolink") reaction that is anabolic, meaning it builds larger, more complex molecules and requires an input of energy along with enzymes.

**Hydrolysis** breaks a polymer into smaller molecules by cleaving the covalent bond between monomers (hydro means water, lysis means break). Water is added to the bond: the H from water goes to one monomer and the OH from water goes to the other. Hydrolysis is catabolic, meaning it breaks larger molecules into smaller ones, and it also uses enzymes. Digestion of food is a real example of hydrolysis at work.

A simple way to keep them straight: synthesis removes water to build, hydrolysis adds water to break.

## How to Use This on the AP Biology Exam

### Written Responses

When a question asks you to describe how a polymer is built or broken, name the reaction and explain what happens to the water. For dehydration synthesis, say a covalent bond forms and the equivalent of a water molecule is removed. For hydrolysis, say water is added and a covalent bond is broken. Always connect the reaction to the monomers and polymer involved.

### Data and Diagrams

You may see diagrams showing monomers joining or a polymer splitting. Look for whether water is being released (synthesis) or added (breaking) to identify the reaction. If the molecule is getting longer, it is dehydration synthesis; if it is getting shorter, it is hydrolysis.

### Common Trap

Use precise terms. Graders look for the exact reaction name and the correct role of water. Saying a molecule simply "breaks apart" without mentioning that water is added will not support a stronger score on a hydrolysis question.

## Common Misconceptions

- "Water is a [product](/ap-bio/unit-3/enzyme-catalysis/study-guide/Jg1jljQ8ZHUvcaKprPGy "fv-autolink") in both reactions." Only dehydration synthesis releases water. Hydrolysis uses water as a reactant.
- "All four macromolecule groups are polymers." Lipids are not true polymers, so they are not assembled and broken down through the same monomer-linking dehydration and hydrolysis reactions as carbohydrates, proteins, and nucleic acids.
- "Dehydration synthesis releases energy because it makes a bond." Building polymers is anabolic and requires an input of energy, not a release.
- "Hydrolysis means water is removed." The name says the opposite. Hydro plus lysis means water is added to break a bond.
- "These reactions happen on their own." Both reactions rely on enzymes to occur at useful rates in cells.

![Diagram showing dehydration synthesis joining monomers by removing water and hydrolysis splitting a polymer by adding water](https://storage.googleapis.com/ug-images-study-guides/legacy-firebase/images/1024px-213_Dehydration_Synthesis_and_Hydrolysis-01.jpg)

###### Image courtesy of WikiMedia Commons.

## Related AP Biology Guides

- [Unit 1 Overview: Chemistry of Life](/ap-bio/unit-1/review/study-guide/NgY7RbUFacBqXbbzx601)
- [1.1 Structure of Water and Hydrogen Bonding](/ap-bio/unit-1/structure-water-hydrogen-bonding/study-guide/bMEKm5Pi5y3Y3XRln0Bj)
- [1.2 Elements of Life](/ap-bio/unit-1/elements-life/study-guide/kLZ8GN081XmAmZpivYFN)
- [1.4 Properties of Biological Macromolecules](/ap-bio/unit-1/properties-biological-macromolecules/study-guide/aAm3FBn4yaR06XhOtFLI)
- [1.5 Lipids](/ap-bio/unit-1/structure-function-biological-macromolecules/study-guide/2Wz7ufs9Bp8zVuceCdg3)
- [1.6 Nucleic Acids](/ap-bio/unit-1/nucleic-acids/study-guide/RKOM4rhL6iJsAMdbDOWU)

## Vocabulary

- **covalent bond**: Chemical bonds formed by the sharing of electrons between atoms, which can be broken or formed during macromolecule reactions.
- **dehydration synthesis**: A chemical reaction that joins two smaller molecules together through covalent bonding by removing water, resulting in the formation of a larger molecule.
- **hydrolysis**: A chemical reaction that breaks down molecules by cleaving covalent bonds through the addition of water, splitting polymers into smaller monomers.
- **monomer**: Small individual molecules that serve as the building blocks for larger polymers.
- **polymer**: A large molecule composed of many monomers linked together through covalent bonds.
- **polymerization**: The process by which many monomers are connected together to form a polymer.

## FAQs

### What are biological macromolecules in AP Biology?

Biological macromolecules are large molecules that make up living things. In this topic, the key idea is how smaller monomers connect into larger polymers in carbohydrates, proteins, and nucleic acids.

### What is the difference between a monomer and a polymer?

A monomer is a small building block molecule, while a polymer is a larger molecule made of many monomers joined by covalent bonds.

### What does dehydration synthesis do?

Dehydration synthesis builds larger molecules by forming a covalent bond between monomers and removing the equivalent of one water molecule.

### What does hydrolysis do?

Hydrolysis breaks a covalent bond between monomers by adding water. The H goes to one piece and the OH goes to the other.

### Are lipids polymers?

Lipids are not true polymers because they are not usually built from repeating monomer chains. They are still biological macromolecules, but their structure is different.

### How should I use dehydration synthesis and hydrolysis on the AP Biology exam?

Identify whether a diagram or prompt is building or breaking a molecule, name the reaction, and explain the role of water in forming or breaking the covalent bond.

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